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VAPB ELISA Kit
VAPB
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details VAPB ELISA Kit
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Target
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VAPB
(VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
Reactivity
All reactivities for VAPB elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human VAP-B ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for VAPB
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Target
See all VAPB ELISA Kits
VAPB
(VAMP (Vesicle-Associated Membrane Protein)-Associated Protein B and C (VAPB))
Alternative Name
VAP-B (VAPB Products )
Synonyms
ALS8 ELISA Kit, VAMP-B ELISA Kit, VAP-B ELISA Kit, AI225786 ELISA Kit, AI840687 ELISA Kit, AI848259 ELISA Kit, D2Abb2e ELISA Kit, R75548 ELISA Kit, VAP33b ELISA Kit, Vamp33b ELISA Kit, fc05d09 ELISA Kit, wu:fa96d05 ELISA Kit, wu:fc05d09 ELISA Kit, zgc:66045 ELISA Kit, vamp-b ELISA Kit, vamp-c ELISA Kit, vap-b ELISA Kit, vap-c ELISA Kit, vapb ELISA Kit, VAMP associated protein B and C ELISA Kit, vesicle-associated membrane protein-associated protein B/C ELISA Kit, vesicle-associated membrane protein, associated protein B and C ELISA Kit, VAMP (vesicle-associated membrane protein)-associated protein B and C ELISA Kit, VAMP (vesicle-associated membrane protein)-associated protein B and C L homeolog ELISA Kit, VAPB ELISA Kit, Tsp_07972 ELISA Kit, Vapb ELISA Kit, vapb ELISA Kit, vapb.L ELISA Kit
Gene ID
9217
UniProt
O95292
Pathways
ER-Nucleus Signaling
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