UGT1A1 ELISA Kit

Catalog No. ABIN4884976

Product Details UGT1A1 ELISA Kit

Target: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human UGT1A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for UGT1A1

Target: UGT1A1 (UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1))

Alternative Name: UGT1A1 (UGT1A1 Products)

Synonyms: BILIQTL1 ELISA Kit, GNT1 ELISA Kit, HUG-BR1 ELISA Kit, UDPGT ELISA Kit, UDPGT 1-1 ELISA Kit, UGT1 ELISA Kit, UGT1A ELISA Kit, Gnt1 ELISA Kit, UGT1A01 ELISA Kit, Udpgt-1a ELISA Kit, UgtBr1 ELISA Kit, Udpgt ELISA Kit, Ugt1 ELISA Kit, zgc:123097 ELISA Kit, UDP glucuronosyltransferase family 1 member A1 ELISA Kit, UDP glucuronosyltransferase 1 family, polypeptide A1 ELISA Kit, UDP-glucuronosyltransferase 1-1 ELISA Kit, UDP-glucuronosyltransferase ELISA Kit, UDP-glucuronosyltransferase 1-6 ELISA Kit, UDP glucuronosyltransferase 1 family polypeptide a1 ELISA Kit, UGT1A1 ELISA Kit, Ugt1a1 ELISA Kit, LOC100065342 ELISA Kit, LOC100125517 ELISA Kit, LOC100229734 ELISA Kit, LOC100405984 ELISA Kit, LOC100511479 ELISA Kit, ugt1a1 ELISA Kit

Gene ID: 54658

UniProt: P22309

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha