Thymopoietin ELISA Kit

Catalog No. ABIN4884832

Product Details Thymopoietin ELISA Kit

Target: Thymopoietin (TMPO)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Thymopoietin (LAP2) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Thymopoietin

Target: Thymopoietin (TMPO)

Alternative Name: Thymopoietin (TMPO Products)

Synonyms: LAP2 ELISA Kit, CMD1T ELISA Kit, LEMD4 ELISA Kit, PRO0868 ELISA Kit, TP ELISA Kit, lap2 ELISA Kit, LAP2beta ELISA Kit, 5630400D24Rik ELISA Kit, AI195756 ELISA Kit, AI606875 ELISA Kit, AW214352 ELISA Kit, AW547477 ELISA Kit, tmpo ELISA Kit, wu:fc26f12 ELISA Kit, wu:fd21f02 ELISA Kit, wu:fi29h05 ELISA Kit, thymopoietin ELISA Kit, thymopoietin S homeolog ELISA Kit, thymopoietin a ELISA Kit, TMPO ELISA Kit, Tmpo ELISA Kit, tmpo.S ELISA Kit, tmpoa ELISA Kit

Gene ID: 7112