×
For best experience we recommend to activate Javascript in your browser.
Transferrin Receptor ELISA Kit
TFRC
Reactivity: Human
Colorimetric
Sandwich ELISA
0.819-200 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details Transferrin Receptor ELISA Kit
(hide)
Target
See all Transferrin Receptor (TFRC) ELISA Kits
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Reactivity
All reactivities for Transferrin Receptor elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.819-200 ng/mL
Minimum Detection Limit
0.819 ng/mL
Application
ELISA
Purpose
Human TfR/Transferrin R/CD71 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human TfR.
Sensitivity
0.81 ng/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling TFRC ELISA Kit
Top Product
Discover our top product TFRC ELISA Kit
Alternatives
(show)
Application Details
(hide)
Application Notes
Recommended Dilution for serum and plasma samples200 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for Transferrin Receptor
(hide)
Target
See all Transferrin Receptor (TFRC) ELISA Kits
Transferrin Receptor (TFRC)
(Transferrin Receptor (p90, CD71) (TFRC))
Alternative Name
TfR (Transferrin R/CD71) (TFRC Products )
Synonyms
Trfr ELISA Kit, LOC100219280 ELISA Kit, tfr ELISA Kit, cd71 ELISA Kit, tfr1 ELISA Kit, trfr ELISA Kit, CD71 ELISA Kit, T9 ELISA Kit, TFR ELISA Kit, TFR1 ELISA Kit, TR ELISA Kit, TRFR ELISA Kit, p90 ELISA Kit, 2610028K12Rik ELISA Kit, AI195355 ELISA Kit, AI426448 ELISA Kit, AU015758 ELISA Kit, E430033M20Rik ELISA Kit, Mtvr-1 ELISA Kit, Mtvr1 ELISA Kit, transferrin receptor ELISA Kit, TFRC ELISA Kit, Tfrc ELISA Kit, tfrc ELISA Kit
Gene ID
7037
UniProt
P02786
Pathways
Transition Metal Ion Homeostasis
Recently viewed
(hide)