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SPRY1 ELISA Kit
SPRY1
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details SPRY1 ELISA Kit
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Target
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SPRY1
(Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human SPRY1 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SPRY1
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Target
See all SPRY1 products
SPRY1
(Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
Alternative Name
SPRY1 (SPRY1 Products )
Synonyms
hSPRY1 ELISA Kit, SPRY1 ELISA Kit, LOC100223875 ELISA Kit, hspry1 ELISA Kit, xspry1 ELISA Kit, sprouty ELISA Kit, sprouty1 ELISA Kit, sprouty-1 ELISA Kit, Xtsprouty1 ELISA Kit, spry1a ELISA Kit, spry1b ELISA Kit, sprouty RTK signaling antagonist 1 ELISA Kit, sprouty RTK signaling antagonist 1 L homeolog ELISA Kit, sprouty RTK signaling antagonist 1 S homeolog ELISA Kit, sprouty homolog 1 (Drosophila) ELISA Kit, SPRY1 ELISA Kit, Spry1 ELISA Kit, spry1 ELISA Kit, spry1.L ELISA Kit, spry1.S ELISA Kit
Gene ID
10252
UniProt
O43609
Pathways
EGFR Signaling Pathway , EGFR Downregulation
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