SMOC2 ELISA Kit

Catalog No. ABIN4884589

Product Details SMOC2 ELISA Kit

Target: SMOC2 (SPARC Related Modular Calcium Binding 2 (SMOC2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human SMOC-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human SMOC-2. Other species not yet determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SMOC2

Target: SMOC2 (SPARC Related Modular Calcium Binding 2 (SMOC2))

Alternative Name: SMOC-2 (SMOC2 Products)

Synonyms: cb488 ELISA Kit, wu:fb75d10 ELISA Kit, zgc:112171 ELISA Kit, SMOC2 ELISA Kit, DTDP1 ELISA Kit, MST117 ELISA Kit, MSTP117 ELISA Kit, MSTP140 ELISA Kit, SMAP2 ELISA Kit, bA270C4A.1 ELISA Kit, bA37D8.1 ELISA Kit, dJ421D16.1 ELISA Kit, 1700056C05Rik ELISA Kit, 5430426J21Rik ELISA Kit, Smoc2l ELISA Kit, SPARC related modular calcium binding 2 ELISA Kit, SPARC-related modular calcium-binding protein 2 ELISA Kit, smoc2 ELISA Kit, SMOC2 ELISA Kit, LOC100512748 ELISA Kit, Smoc2 ELISA Kit

Gene ID: 64094

UniProt: Q9H3U7