SMAD4 ELISA Kit

Catalog No. ABIN4884583

Product Details SMAD4 ELISA Kit

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human SMAD4 ELISA Kit for Cell Culture Supernatants.

Sample Type: Cell Culture Supernatant

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human SMAD4.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: Smad4 (SMAD4 Products)

Synonyms: smad4 ELISA Kit, DPC4 ELISA Kit, JIP ELISA Kit, MADH4 ELISA Kit, MYHRS ELISA Kit, AW743858 ELISA Kit, D18Wsu70e ELISA Kit, Madh4 ELISA Kit, XSmad4alpha ELISA Kit, Xsmad4 ELISA Kit, dpc4 ELISA Kit, jip ELISA Kit, madh4 ELISA Kit, xsmad4a ELISA Kit, SMAD family member 4 ELISA Kit, Smad4 protein ELISA Kit, SMAD family member 4, gene 1 L homeolog ELISA Kit, SMAD4 ELISA Kit, smad4 ELISA Kit, Smad4 ELISA Kit, smad4.1.L ELISA Kit

Gene ID: 4089

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy