SH2D1A ELISA Kit

Catalog No. ABIN4884511

Product Details SH2D1A ELISA Kit

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human SH2D1A ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SH2D1A

Target: SH2D1A (SH2 Domain Containing 1A (SH2D1A))

Alternative Name: SH2D1A (SH2D1A Products)

Synonyms: DSHP ELISA Kit, EBVS ELISA Kit, IMD5 ELISA Kit, LYP ELISA Kit, MTCP1 ELISA Kit, SAP ELISA Kit, SAP/SH2D1A ELISA Kit, XLP ELISA Kit, XLPD ELISA Kit, RGD1562408 ELISA Kit, Gm686 ELISA Kit, SH2D1A ELISA Kit, SH2 domain containing 1A ELISA Kit, SH2D1A ELISA Kit, Sh2d1a ELISA Kit

Gene ID: 4068

UniProt: O60880

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process