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SERPIND1 ELISA Kit
SERPIND1
Reactivity: Human
Colorimetric
Sandwich ELISA
1.23-300 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details SERPIND1 ELISA Kit
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Target
See all SERPIND1 ELISA Kits
SERPIND1
(serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
Reactivity
All reactivities for SERPIND1 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
1.23-300 ng/mL
Minimum Detection Limit
1.23 ng/mL
Application
ELISA
Purpose
Human SERPIND1 (Heparin Cofactor II) ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects human Serpin D1. Other species not determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples20,000 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPIND1
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Target
See all SERPIND1 ELISA Kits
SERPIND1
(serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))
Alternative Name
SERPIND1///Heparin Cofactor II (SERPIND1 Products )
Synonyms
D22S673 ELISA Kit, HC2 ELISA Kit, HCF2 ELISA Kit, HCII ELISA Kit, HLS2 ELISA Kit, LS2 ELISA Kit, THPH10 ELISA Kit, serpind1 ELISA Kit, MGC53936 ELISA Kit, rls2var1 ELISA Kit, AA985900 ELISA Kit, AI303446 ELISA Kit, Hcf2 ELISA Kit, serpin family D member 1 ELISA Kit, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog ELISA Kit, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade D, member 1 ELISA Kit, SERPIND1 ELISA Kit, serpind1.L ELISA Kit, Serpind1 ELISA Kit, serpind1 ELISA Kit
Gene ID
3053
UniProt
P05546
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