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SERPINB8 ELISA Kit
SERPINB8
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details SERPINB8 ELISA Kit
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Target
See all SERPINB8 ELISA Kits
SERPINB8
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 8 (SERPINB8))
Reactivity
All reactivities for SERPINB8 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Serpin B8 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for SERPINB8
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Target
See all SERPINB8 ELISA Kits
SERPINB8
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 8 (SERPINB8))
Alternative Name
Serpin B8 (SERPINB8 Products )
Synonyms
pi8 ELISA Kit, cap2 ELISA Kit, CAP-2 ELISA Kit, CAP2 ELISA Kit, NK10 ELISA Kit, Spi8 ELISA Kit, ovalbumin ELISA Kit, PI8 ELISA Kit, serpin family B member 8 ELISA Kit, serpin B8 ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 8 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 8 ELISA Kit, Serpinb8 ELISA Kit, SERPINB8 ELISA Kit, LOC607205 ELISA Kit, serpinb8 ELISA Kit, LOC100424017 ELISA Kit
Gene ID
5271
UniProt
P50452
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