ROR2 ELISA Kit

Catalog No. ABIN4884377

Product Details ROR2 ELISA Kit

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human ROR2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ROR2

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Alternative Name: ROR2 (ROR2 Products)

Synonyms: BDB ELISA Kit, BDB1 ELISA Kit, NTRKR2 ELISA Kit, Ntrkr2 ELISA Kit, mRor2 ELISA Kit, ROR2 ELISA Kit, bdb ELISA Kit, bdb1 ELISA Kit, Xror2 ELISA Kit, ntrkr2 ELISA Kit, MGC97773 ELISA Kit, LOC100219935 ELISA Kit, ror2 ELISA Kit, xror2 ELISA Kit, receptor tyrosine kinase like orphan receptor 2 ELISA Kit, receptor tyrosine kinase-like orphan receptor 2 ELISA Kit, receptor tyrosine kinase like orphan receptor 2 L homeolog ELISA Kit, ROR2 ELISA Kit, Ror2 ELISA Kit, ror2 ELISA Kit, ror2.L ELISA Kit

Gene ID: 4920

UniProt: Q01974

Pathways: RTK Signaling, WNT Signaling