ROBO3 ELISA Kit

Catalog No. ABIN4884368

Product Details ROBO3 ELISA Kit

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15-0.06 ng/mL

Minimum Detection Limit: 15 ng/mL

Application: ELISA

Purpose: Human ROBO3 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human ROBO3

Sensitivity: 0.06 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ROBO3

Target: ROBO3 (Roundabout, Axon Guidance Receptor, Homolog 3 (ROBO3))

Alternative Name: ROBO3 (ROBO3 Products)

Synonyms: HGPPS ELISA Kit, HGPS ELISA Kit, RBIG1 ELISA Kit, RIG1 ELISA Kit, CG14345 ELISA Kit, CT17158 ELISA Kit, Robo-3 ELISA Kit, Robo3 ELISA Kit, dRobo-3 ELISA Kit, DmelCG5423 ELISA Kit, CG5423 ELISA Kit, ROBO3 ELISA Kit, robo3 ELISA Kit, DKFZp468G0223 ELISA Kit, Rbig1 ELISA Kit, Rig-1 ELISA Kit, Rig1 ELISA Kit, Robo3a ELISA Kit, Robo3b ELISA Kit, cb503 ELISA Kit, roundabout guidance receptor 3 ELISA Kit, roundabout 3 ELISA Kit, robo3 ELISA Kit, roundabout guidance receptor 3 L homeolog ELISA Kit, roundabout, axon guidance receptor, homolog 3 (Drosophila) ELISA Kit, ROBO3 ELISA Kit, robo3 ELISA Kit, Robo3 ELISA Kit, LOC658818 ELISA Kit, robo3.L ELISA Kit

Gene ID: 64221

UniProt: Q96MS0