PTH1R ELISA Kit

Catalog No. ABIN4884292

Product Details PTH1R ELISA Kit

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 8.19-2.000 pg/mL

Minimum Detection Limit: 8.19 pg/mL

Application: ELISA

Purpose: Human PTH1R ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human PTH1R.

Sensitivity: 8.2 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PTH1R

Target: PTH1R (Parathyroid Hormone 1 Receptor (PTH1R))

Alternative Name: PTH1R (PTH1R Products)

Synonyms: PFE ELISA Kit, PTHR ELISA Kit, PTHR1 ELISA Kit, PPR ELISA Kit, Pthr ELISA Kit, Pthr1 ELISA Kit, pthr1 ELISA Kit, zPTH1R ELISA Kit, PTHrel ELISA Kit, PTH1 ELISA Kit, PTH ELISA Kit, parathyroid hormone 1 receptor ELISA Kit, parathyroid hormone 1 receptor a ELISA Kit, PTH1R ELISA Kit, Pth1r ELISA Kit, pth1ra ELISA Kit

Gene ID: 5745

UniProt: Q03431

Pathways: Regulation of Carbohydrate Metabolic Process