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PSMA1 ELISA Kit
PSMA1
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details PSMA1 ELISA Kit
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Target
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PSMA1
(Proteasome Subunit alpha Type 1 (PSMA1))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human PSMA1 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PSMA1
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Target
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PSMA1
(Proteasome Subunit alpha Type 1 (PSMA1))
Alternative Name
PSMA1 (PSMA1 Products )
Synonyms
PSMA1 ELISA Kit, pros30 ELISA Kit, DDBDRAFT_0204226 ELISA Kit, DDBDRAFT_0214956 ELISA Kit, DDB_0204226 ELISA Kit, DDB_0214956 ELISA Kit, C2 ELISA Kit, HC2 ELISA Kit, Pros-30 ELISA Kit, alpha-type ELISA Kit, NU ELISA Kit, PROS30 ELISA Kit, CC2 ELISA Kit, zgc:92726 ELISA Kit, 143314_at ELISA Kit, Alpha-6 ELISA Kit, CG4904 ELISA Kit, Dmel\CG4904 ELISA Kit, PROS-35 ELISA Kit, PROS-Dm35 ELISA Kit, Pros-35 ELISA Kit, Pros-Dm35 ELISA Kit, Prosalpha6 ELISA Kit, alpha6 ELISA Kit, alpha_dm ELISA Kit, anon-SAGE:Wang-116 ELISA Kit, pros 35 ELISA Kit, pros35 ELISA Kit, proteasome subunit alpha 1 ELISA Kit, proteasome subunit alpha type 1 ELISA Kit, 20S proteasome subunit C2 ELISA Kit, proteasome (prosome, macropain) subunit, alpha type 1 ELISA Kit, proteasome subunit alpha 1 L homeolog ELISA Kit, Proteasome alpha6 subunit ELISA Kit, PSMA1 ELISA Kit, psma1 ELISA Kit, CNB05540 ELISA Kit, psmA1 ELISA Kit, Psma1 ELISA Kit, psma1.L ELISA Kit, Prosalpha6 ELISA Kit
Gene ID
5682
UniProt
P25786
Pathways
Mitotic G1-G1/S Phases , DNA Replication , Synthesis of DNA
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