Presenilin 2 ELISA Kit

Catalog No. ABIN4884274

Product Details Presenilin 2 ELISA Kit

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Presenilin-2 (PSEN2) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Presenilin-2 (PSEN2)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Presenilin 2

Target: Presenilin 2 (PSEN2) (Presenilin 2 (Alzheimer Disease 4) (PSEN2))

Alternative Name: Presenilin 2 (PSEN2 Products)

Synonyms: AD3L ELISA Kit, AD4 ELISA Kit, CMD1V ELISA Kit, PS2 ELISA Kit, STM2 ELISA Kit, ALG-3 ELISA Kit, Ad4h ELISA Kit, Alg3 ELISA Kit, PS-2 ELISA Kit, Psnl2 ELISA Kit, pre2 ELISA Kit, zf-PS2 ELISA Kit, PSNL2 ELISA Kit, PSEN2 ELISA Kit, ad4 ELISA Kit, ps2 ELISA Kit, ad3l ELISA Kit, stm2 ELISA Kit, Ps2 ELISA Kit, presenilin 2 ELISA Kit, presenilin 2 S homeolog ELISA Kit, presenilin 2 (Alzheimer disease 4) ELISA Kit, trefoil factor 1 ELISA Kit, PSEN2 ELISA Kit, Psen2 ELISA Kit, psen2 ELISA Kit, psen2.S ELISA Kit, Tff1 ELISA Kit

Gene ID: 5664

UniProt: P49810

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway