OCT4 ELISA Kit

Catalog No. ABIN4884033

Product Details OCT4 ELISA Kit

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.20-50 ng/mL

Minimum Detection Limit: 0.20 ng/mL

Application: ELISA

Purpose: Human OCT-4 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-3 (BMPR-IA), ALK-6 (BMPR-IB), Brachyury, CD38, E-Cadherein, GATA4, hCGbeta, Nanog, Nestin, PDX-1 (PF-1), SOX17, SOX2, VEGFR2

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: OCT-4 (POU5F1 Products)

Synonyms: OCT3 ELISA Kit, OCT4 ELISA Kit, OTF-3 ELISA Kit, OTF3 ELISA Kit, OTF4 ELISA Kit, Oct-3 ELISA Kit, Oct-4 ELISA Kit, POU5F1 ELISA Kit, gp9 ELISA Kit, gp-9 ELISA Kit, oct4 ELISA Kit, pou2 ELISA Kit, POU-2 ELISA Kit, cb197 ELISA Kit, spg/pou2 ELISA Kit, chunp6868 ELISA Kit, wu:fd18d06 ELISA Kit, NF-A3 ELISA Kit, Oct-3/4 ELISA Kit, Oct3 ELISA Kit, Oct3/4 ELISA Kit, Oct4 ELISA Kit, Otf-3 ELISA Kit, Otf-4 ELISA Kit, Otf3 ELISA Kit, Otf3-rs7 ELISA Kit, Otf3g ELISA Kit, Otf4 ELISA Kit, POU class 5 homeobox 1 ELISA Kit, POU domain, class 5, transcription factor 3 ELISA Kit, POU domain, class 5, transcription factor 1 ELISA Kit, POU5F1 ELISA Kit, pou5f3 ELISA Kit, Pou5f1 ELISA Kit

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance