OPCML ELISA Kit

Catalog No. ABIN4884030

Product Details OPCML ELISA Kit

Target: OPCML (Opioid Binding Protein/cell Adhesion Molecule-Like (OPCML))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human OBCAM (OPCML) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for OPCML

Target: OPCML (Opioid Binding Protein/cell Adhesion Molecule-Like (OPCML))

Alternative Name: OBCAM (OPCML Products)

Synonyms: IGLON1 ELISA Kit, OBCAM ELISA Kit, OPCM ELISA Kit, 2900075O15Rik ELISA Kit, 3732419F12 ELISA Kit, AI844366 ELISA Kit, B930023M13Rik ELISA Kit, C230027C17 ELISA Kit, Gm181 ELISA Kit, Obcam ELISA Kit, opioid binding protein/cell adhesion molecule like ELISA Kit, opioid binding protein/cell adhesion molecule-like ELISA Kit, OPCML ELISA Kit, Opcml ELISA Kit

Gene ID: 4978

UniProt: Q14982