NPTXR ELISA Kit

Catalog No. ABIN4883974

Product Details NPTXR ELISA Kit

Target: NPTXR (Neuronal Pentraxin Receptor (NPTXR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.410-100 ng/mL

Minimum Detection Limit: 0.410 ng/mL

Application: ELISA

Purpose: Human NPTXR ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human NPTXR.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for NPTXR

Target: NPTXR (Neuronal Pentraxin Receptor (NPTXR))

Alternative Name: NPTXR (NPTXR Products)

Synonyms: NPR ELISA Kit, 1200009K17Rik ELISA Kit, 1700036C17Rik ELISA Kit, 5730406O18Rik ELISA Kit, AI452369 ELISA Kit, AI785356 ELISA Kit, D15Bwg0580e ELISA Kit, Npcd ELISA Kit, Npr ELISA Kit, npr ELISA Kit, nptxr ELISA Kit, si:dkeyp-20e4.1 ELISA Kit, zgc:158178 ELISA Kit, neuronal pentraxin receptor ELISA Kit, neuronal pentraxin receptor L homeolog ELISA Kit, neuronal pentraxin receptor b ELISA Kit, NPTXR ELISA Kit, Nptxr ELISA Kit, nptxr.L ELISA Kit, nptxrb ELISA Kit

Gene ID: 23467

UniProt: O95502