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NLRP2 ELISA Kit
NLRP2
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details NLRP2 ELISA Kit
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Target
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NLRP2
(NLR Family, Pyrin Domain Containing 2 (NLRP2))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human NLRP2/NALP2 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for NLRP2
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Target
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NLRP2
(NLR Family, Pyrin Domain Containing 2 (NLRP2))
Alternative Name
NLRP2 (NLRP2 Products )
Synonyms
CLR19.9 ELISA Kit, NALP2 ELISA Kit, NBS1 ELISA Kit, PAN1 ELISA Kit, PYPAF2 ELISA Kit, NLRP2 ELISA Kit, E330007A02Rik ELISA Kit, Nalp2 ELISA Kit, NLR family pyrin domain containing 2 ELISA Kit, NACHT, LRR and PYD domains-containing protein 2 ELISA Kit, NLR family pyrin domain containing 7 ELISA Kit, NLR family, pyrin domain containing 2 ELISA Kit, NLRP2 ELISA Kit, LOC100301026 ELISA Kit, LOC100387349 ELISA Kit, NLRP7 ELISA Kit, Nlrp2 ELISA Kit
Gene ID
55655
UniProt
Q9NX02
Pathways
Production of Molecular Mediator of Immune Response , Positive Regulation of Endopeptidase Activity , Inflammasome
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