PVRL1 ELISA Kit

Catalog No. ABIN4883868

Product Details PVRL1 ELISA Kit

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Nectin-1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes human Nectin-1. Other species not yet determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PVRL1

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Alternative Name: Nectin-1 (PVRL1 Products)

Synonyms: CD111 ELISA Kit, CLPED1 ELISA Kit, ED4 ELISA Kit, HIgR ELISA Kit, HVEC ELISA Kit, OFC7 ELISA Kit, PRR ELISA Kit, PRR1 ELISA Kit, PVRR ELISA Kit, PVRR1 ELISA Kit, SK-12 ELISA Kit, nectin-1 ELISA Kit, prr1 ELISA Kit, PVRL1 ELISA Kit, pvrl1 ELISA Kit, AI835281 ELISA Kit, AW549174 ELISA Kit, Cd111 ELISA Kit, HveC ELISA Kit, cd111 ELISA Kit, cd112 ELISA Kit, clped1 ELISA Kit, ed4 ELISA Kit, higr ELISA Kit, hveb ELISA Kit, hvec ELISA Kit, nectin-2 ELISA Kit, ofc7 ELISA Kit, prr ELISA Kit, prr2 ELISA Kit, pvrl2 ELISA Kit, pvrl2.L ELISA Kit, pvrr ELISA Kit, pvrr1 ELISA Kit, pvrr2 ELISA Kit, sk-12 ELISA Kit, nectin cell adhesion molecule 1 ELISA Kit, nectin-1 ELISA Kit, Poliovirus receptor-related protein 1 ELISA Kit, poliovirus receptor-related 1 (herpesvirus entry mediator C) ELISA Kit, nectin cell adhesion molecule 2 L homeolog ELISA Kit, NECTIN1 ELISA Kit, LOC100145553 ELISA Kit, pvrl1 ELISA Kit, nectin1 ELISA Kit, Nectin1 ELISA Kit, PVRL1 ELISA Kit, nectin2.L ELISA Kit

Gene ID: 5818

UniProt: Q15223

Pathways: Cell-Cell Junction Organization