Macrophage Mannose Receptor 1 ELISA Kit
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- Target See all Macrophage Mannose Receptor 1 (MRC1) ELISA Kits
- Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.41-100 ng/mL
- Minimum Detection Limit
- 0.41 ng/mL
- Application
- ELISA
- Purpose
- Human MMR (CD206/MRC1) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- The antibody pair provided in this kit recognizes Human Macrophage Mannose Receptor (MRC1 / CD206)
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Application Notes
- Recommended Dilution for serum and plasma samples30 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all Macrophage Mannose Receptor 1 (MRC1) ELISA Kits
- Macrophage Mannose Receptor 1 (MRC1) (Mannose Receptor, C Type 1 (MRC1))
- Alternative Name
- MMR (MRC1 Products)
- Synonyms
- CD206 ELISA Kit, CLEC13D ELISA Kit, CLEC13DL ELISA Kit, MMR ELISA Kit, MRC1L1 ELISA Kit, bA541I19.1 ELISA Kit, AW259686 ELISA Kit, MR ELISA Kit, mrc1 ELISA Kit, MGC86525 ELISA Kit, MRC1 ELISA Kit, MRC1L-A ELISA Kit, mannose receptor C-type 1 ELISA Kit, mannose receptor, C type 1 ELISA Kit, mannose receptor C-type 1 L homeolog ELISA Kit, mediator of replication checkpoint 1 ELISA Kit, MRC1 ELISA Kit, Mrc1 ELISA Kit, mrc1.L ELISA Kit, mrc1 ELISA Kit
- Gene ID
- 4360
- UniProt
- P22897
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