IL17RD ELISA Kit

Catalog No. ABIN4883328

Product Details IL17RD ELISA Kit

Target: IL17RD (Interleukin 17 Receptor D (IL17RD))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human IL-17 RD ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes human IL-17 RD. Other species not yet determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IL17RD

Target: IL17RD (Interleukin 17 Receptor D (IL17RD))

Alternative Name: IL-17 RD (IL17RD Products)

Synonyms: IL17RD ELISA Kit, sef ELISA Kit, il-17rd ELISA Kit, il17rlm ELISA Kit, HH18 ELISA Kit, IL-17RD ELISA Kit, IL17RLM ELISA Kit, SEF ELISA Kit, 2810004A10Rik ELISA Kit, AI428510 ELISA Kit, Sef ELISA Kit, Sef-S ELISA Kit, RGD1565237 ELISA Kit, cb208 ELISA Kit, id:ibd2875 ELISA Kit, id:ibd5059 ELISA Kit, zgc:136328 ELISA Kit, interleukin 17 receptor D ELISA Kit, IL17RD ELISA Kit, il17rd ELISA Kit, Il17rd ELISA Kit

Gene ID: 54756

UniProt: Q8NFM7

Pathways: Toll-Like Receptors Cascades