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HS3ST1 ELISA Kit

HS3ST1 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4883221
  • Target See all HS3ST1 ELISA Kits
    HS3ST1 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 1 (HS3ST1))
    Reactivity
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    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human HS3ST1 ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes .
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product HS3ST1 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all HS3ST1 ELISA Kits
    HS3ST1 (Heparan Sulfate (Glucosamine) 3-O-Sulfotransferase 1 (HS3ST1))
    Alternative Name
    HS3ST1 (HS3ST1 Products)
    Synonyms
    3OST1 ELISA Kit, 3OST ELISA Kit, 3-Ost ELISA Kit, D5Wsu110e ELISA Kit, Hsg3ost ELISA Kit, heparan sulfate-glucosamine 3-sulfotransferase 1 L homeolog ELISA Kit, heparan sulfate-glucosamine 3-sulfotransferase 1 ELISA Kit, heparan sulfate (glucosamine) 3-O-sulfotransferase 1 ELISA Kit, hs3st1.L ELISA Kit, HS3ST1 ELISA Kit, hs3st1 ELISA Kit, Hs3st1 ELISA Kit
    Gene ID
    9957
    UniProt
    O14792
    Pathways
    Glycosaminoglycan Metabolic Process
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