GLI3 ELISA Kit

Catalog No. ABIN4883079

Product Details GLI3 ELISA Kit

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GLI-3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human GLI-3.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GLI3

Target: GLI3 (GLI Family Zinc Finger 3 (GLI3))

Alternative Name: GLI-3 (GLI3 Products)

Synonyms: ACLS ELISA Kit, GCPS ELISA Kit, GLI3-190 ELISA Kit, GLI3FL ELISA Kit, PAP-A ELISA Kit, PAPA ELISA Kit, PAPA1 ELISA Kit, PAPB ELISA Kit, PHS ELISA Kit, PPDIV ELISA Kit, xgli3 ELISA Kit, AI854843 ELISA Kit, AU023367 ELISA Kit, Bph ELISA Kit, Pdn ELISA Kit, Xt ELISA Kit, add ELISA Kit, im:7142603 ELISA Kit, GLI3 ELISA Kit, GLI family zinc finger 3 ELISA Kit, GLI family zinc finger 3 L homeolog ELISA Kit, GLI-Kruppel family member GLI3 ELISA Kit, GLI3 ELISA Kit, Gli3 ELISA Kit, gli3.L ELISA Kit, gli3 ELISA Kit

Gene ID: 2737

UniProt: P10071

Pathways: Hedgehog Signaling