GFRA2 ELISA Kit

Catalog No. ABIN4883060

Product Details GFRA2 ELISA Kit

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human GFR alpha-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human GFR alpha-2. Other species not yet determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GFRA2

Target: GFRA2 (GDNF Family Receptor alpha 2 (GFRA2))

Alternative Name: GFR alpha-2 (GFRA2 Products)

Synonyms: GDNFRB ELISA Kit, NRTNR-ALPHA ELISA Kit, NTNRA ELISA Kit, RETL2 ELISA Kit, TRNR2 ELISA Kit, Retl2 ELISA Kit, NTNRALPHA ELISA Kit, ntnra ELISA Kit, retl2 ELISA Kit, trnr2 ELISA Kit, gdnfrb ELISA Kit, nrtnr-alpha ELISA Kit, gfra2 ELISA Kit, GDNF family receptor alpha 2 ELISA Kit, glial cell line derived neurotrophic factor family receptor alpha 2 ELISA Kit, GDNF family receptor alpha 2a ELISA Kit, GFRA2 ELISA Kit, Gfra2 ELISA Kit, gfra2 ELISA Kit, gfra2a ELISA Kit

Gene ID: 2675

UniProt: O00451