GATA2 ELISA Kit

Catalog No. ABIN4883020

Product Details GATA2 ELISA Kit

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human GATA-2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA-2 (GATA2 Products)

Synonyms: DCML ELISA Kit, MONOMAC ELISA Kit, NFE1B ELISA Kit, Gata-2 ELISA Kit, xgata-2 ELISA Kit, xgata2 ELISA Kit, GATA-2 ELISA Kit, NF-E1b ELISA Kit, GATA2 ELISA Kit, Gata2 ELISA Kit, gata2 ELISA Kit, zg2 ELISA Kit, zgc:91840 ELISA Kit, GATA binding protein 2 ELISA Kit, GATA binding protein 2 L homeolog ELISA Kit, amidase ELISA Kit, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A ELISA Kit, hypothetical protein ELISA Kit, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) ELISA Kit, GATA2 transcription factor ELISA Kit, putative gata binding factor ELISA Kit, GATA binding protein 2a ELISA Kit, GATA binding protein 2b ELISA Kit, GATA2 ELISA Kit, Gata2 ELISA Kit, gata2.L ELISA Kit, RR_RS20245 ELISA Kit, gatA2 ELISA Kit, Smp_018590 ELISA Kit, gata2a ELISA Kit, gata2b ELISA Kit

Gene ID: 2624

UniProt: P23769

Pathways: Stem Cell Maintenance