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WFIKKN2 ELISA Kit
WFIKKN2
Reactivity: Human
Colorimetric
Sandwich ELISA
20-5000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details WFIKKN2 ELISA Kit
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Target
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WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Reactivity
All reactivities for WFIKKN2 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
20-5000 pg/mL
Minimum Detection Limit
20 pg/mL
Application
ELISA
Purpose
Human GASP-1 (WFIKKNRP) ELISA Kit for serum, plasma, and cell culture supernatant samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples10 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for WFIKKN2
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Target
See all WFIKKN2 ELISA Kits
WFIKKN2
(WAP, Follistatin/kazal, Immunoglobulin, Kunitz and Netrin Domain Containing 2 (WFIKKN2))
Alternative Name
GASP-1///WFIKKNRP (WFIKKN2 Products )
Synonyms
GASP-1 ELISA Kit, WFDC20B ELISA Kit, WFIKKNRP ELISA Kit, hGASP-1 ELISA Kit, 2610304F08Rik ELISA Kit, AY100450 ELISA Kit, Gasp1 ELISA Kit, WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 ELISA Kit, WFIKKN2 ELISA Kit, Wfikkn2 ELISA Kit
Gene ID
9737
UniProt
Q5JY77
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