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FOLR3 ELISA Kit

FOLR3 Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4882917
  • Target See all FOLR3 ELISA Kits
    FOLR3 (Folate Receptor 3 (Gamma) (FOLR3))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human FOLR3 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human FOLR3.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all FOLR3 ELISA Kits
    FOLR3 (Folate Receptor 3 (Gamma) (FOLR3))
    Alternative Name
    FOLR3 (FOLR3 Products)
    Synonyms
    FR-G ELISA Kit, FR-gamma ELISA Kit, gamma-hFR ELISA Kit, folate receptor 3 ELISA Kit, folate receptor gamma ELISA Kit, FOLR3 ELISA Kit, LOC748291 ELISA Kit
    Gene ID
    2352
    UniProt
    P41439
    Pathways
    Dicarboxylic Acid Transport
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