EXTL3 ELISA Kit

Catalog No. ABIN4882781

Product Details EXTL3 ELISA Kit

Target: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Exostosin-like 3/EXTL3 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TRP14.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for EXTL3

Target: EXTL3 (Exostoses (Multiple)-Like 3 (EXTL3))

Alternative Name: Exostosin-Like 3 (EXTL3 Products)

Synonyms: BcDNA:LD21192 ELISA Kit, Botv ELISA Kit, CG15110 ELISA Kit, DEXT3 ELISA Kit, DEXTL3 ELISA Kit, Dextl3 ELISA Kit, Dmel\\CG15110 ELISA Kit, Extl3 ELISA Kit, 2900009G18Rik ELISA Kit, AU040105 ELISA Kit, Ext1l ELISA Kit, mKIAA0519 ELISA Kit, BOTV ELISA Kit, EXTL1L ELISA Kit, EXTR1 ELISA Kit, REGR ELISA Kit, RPR ELISA Kit, Regr ELISA Kit, brother of tout-velu ELISA Kit, exostoses (multiple)-like 3 ELISA Kit, exostosin like glycosyltransferase 3 ELISA Kit, exostosin-like glycosyltransferase 3 ELISA Kit, botv ELISA Kit, Extl3 ELISA Kit, EXTL3 ELISA Kit

Gene ID: 2137

UniProt: O43909

Pathways: Glycosaminoglycan Metabolic Process, ER-Nucleus Signaling