ETS1 ELISA Kit

Catalog No. ABIN4882778

Product Details ETS1 ELISA Kit

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Ets-1/p54 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human TREML1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ETS1

Target: ETS1 (V-Ets erythroblastosis Virus E26 Oncogene Homolog 1 (Avian) (ETS1))

Alternative Name: Ets-1 (ETS1 Products)

Synonyms: ETS-1 ELISA Kit, EWSR2 ELISA Kit, ets1a-a ELISA Kit, XE1-a ELISA Kit, X1-c-ets-1a ELISA Kit, ETS-1A ELISA Kit, ETS-1B ELISA Kit, TNIP1 ELISA Kit, c-ets-1 ELISA Kit, c-ets1 ELISA Kit, Ets-1 ELISA Kit, Etsoncb ELISA Kit, Tpl1 ELISA Kit, ets1 ELISA Kit, ets ELISA Kit, AI196000 ELISA Kit, AI448617 ELISA Kit, D230050P06 ELISA Kit, cb516 ELISA Kit, id:ibd1116 ELISA Kit, zgc:110573 ELISA Kit, XE1-b ELISA Kit, ets-1 ELISA Kit, ets1-B ELISA Kit, ETS proto-oncogene 1, transcription factor ELISA Kit, v-ets avian erythroblastosis virus E26 oncogene homolog 1 S homeolog ELISA Kit, v-ets avian erythroblastosis virus E26 oncogene homolog 1 ELISA Kit, v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) ELISA Kit, E26 avian leukemia oncogene 1, 5' domain ELISA Kit, v-ets avian erythroblastosis virus E26 oncogene homolog 1 L homeolog ELISA Kit, ETS1 ELISA Kit, ets1.S ELISA Kit, Ets1 ELISA Kit, ets1 ELISA Kit, ets1.L ELISA Kit

Gene ID: 2113

UniProt: P14921