Dystroglycan ELISA Kit

Catalog No. ABIN4882681

Product Details Dystroglycan ELISA Kit

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Dystroglycan ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human SorCS1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: Dystroglycan (DAG1 Products)

Synonyms: LOC398500 ELISA Kit, dag1 ELISA Kit, MGC53537 ELISA Kit, 156DAG ELISA Kit, A3a ELISA Kit, AGRNR ELISA Kit, DAG ELISA Kit, MDDGC7 ELISA Kit, MDDGC9 ELISA Kit, DAG1 ELISA Kit, RAB7 ELISA Kit, dg ELISA Kit, a3a ELISA Kit, dag ELISA Kit, agrnr ELISA Kit, 156dag ELISA Kit, D9Wsu13e ELISA Kit, DG ELISA Kit, Dp427 ELISA Kit, Dp71 ELISA Kit, CG18250 ELISA Kit, CT41273 ELISA Kit, DmDG ELISA Kit, Dmel\\CG18250 ELISA Kit, atu ELISA Kit, dgn ELISA Kit, dys ELISA Kit, GB14967 ELISA Kit, APOJ ELISA Kit, CLI ELISA Kit, RATTRPM2B ELISA Kit, SGP-2 ELISA Kit, SGP2 ELISA Kit, SP-40 ELISA Kit, SP40 ELISA Kit, TRPM-2 ELISA Kit, TRPM2B ELISA Kit, Trpm2 ELISA Kit, Trpmb ELISA Kit, Ala-1 ELISA Kit, H9/25 ELISA Kit, Ly-27 ELISA Kit, Ly-6 ELISA Kit, Ly27 ELISA Kit, wu:fb83d06 ELISA Kit, wu:fi25f06 ELISA Kit, wu:fi37b08 ELISA Kit, zgc:109786 ELISA Kit, DystroGlycaN ELISA Kit, dystroglycan 1 L homeolog ELISA Kit, dystroglycan 1 ELISA Kit, dystroglycan 1 S homeolog ELISA Kit, Dystroglycan ELISA Kit, dystroglycan ELISA Kit, clusterin ELISA Kit, lymphocyte antigen 6 complex ELISA Kit, dgn-1 ELISA Kit, dag1.L ELISA Kit, dgn-2 ELISA Kit, dgn-3 ELISA Kit, DAG1 ELISA Kit, dag1.S ELISA Kit, Dag1 ELISA Kit, dag1 ELISA Kit, Dg ELISA Kit, LOC408826 ELISA Kit, Clu ELISA Kit, Ly6 ELISA Kit

Gene ID: 1605

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus