DSCAM ELISA Kit

Catalog No. ABIN4882660

Product Details DSCAM ELISA Kit

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human DSCAM ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for DSCAM

Target: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Alternative Name: DSCAM (DSCAM Products)

Synonyms: CHD2-42 ELISA Kit, CHD2-52 ELISA Kit, Dscam ELISA Kit, DSCAM ELISA Kit, chd2-42 ELISA Kit, chd2-52 ELISA Kit, dscam ELISA Kit, GB15141 ELISA Kit, 4932410A21Rik ELISA Kit, 43Bc ELISA Kit, CG17800 ELISA Kit, CT39257 ELISA Kit, DScam ELISA Kit, DmDscam ELISA Kit, Dm_2R:13612 ELISA Kit, Dmel\\CG17800 ELISA Kit, Dscam-hv ELISA Kit, Dscam1 ELISA Kit, FBgn0033159 ELISA Kit, Neu1 ELISA Kit, dScam ELISA Kit, l(2)05518 ELISA Kit, l(2)43Bc ELISA Kit, p270 ELISA Kit, DS cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule a ELISA Kit, Down syndrome cell adhesion molecule 1 ELISA Kit, Down syndrome cell adhesion molecule-like protein Dscam2 ELISA Kit, down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule L homeolog ELISA Kit, DSCAM ELISA Kit, Dscam ELISA Kit, dscam ELISA Kit, dscama ELISA Kit, Dscam1 ELISA Kit, LOC5573626 ELISA Kit, CpipJ_CPIJ006173 ELISA Kit, dscam.L ELISA Kit

Gene ID: 1826

UniProt: O60469

Pathways: Regulation of Cell Size