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DR3/LARD ELISA Kit
TNFRSF25
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details DR3/LARD ELISA Kit
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Target
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DR3/LARD (TNFRSF25)
(Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))
Reactivity
All reactivities for DR3/LARD elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human Death Receptor 3 (TNFRSF25) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Human Death Receptor 3 (TNFRSF25)
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for DR3/LARD
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Target
See all DR3/LARD (TNFRSF25) ELISA Kits
DR3/LARD (TNFRSF25)
(Tumor Necrosis Factor Receptor Superfamily, Member 25 (TNFRSF25))
Alternative Name
DR3 (TNFRSF25 Products )
Synonyms
APO-3 ELISA Kit, DDR3 ELISA Kit, DR3 ELISA Kit, LARD ELISA Kit, TNFRSF12 ELISA Kit, TR3 ELISA Kit, TRAMP ELISA Kit, WSL-1 ELISA Kit, WSL-LR ELISA Kit, Tnfrsf12 ELISA Kit, Wsl ELISA Kit, TNFRSF25 ELISA Kit, TNF receptor superfamily member 25 ELISA Kit, tumor necrosis factor receptor superfamily, member 25 ELISA Kit, TNFRSF25 ELISA Kit, Tnfrsf25 ELISA Kit
Gene ID
8718
UniProt
Q93038
Pathways
Apoptosis
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