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DPP7 ELISA Kit
DPP7
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details DPP7 ELISA Kit
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Target
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DPP7
(Dipeptidyl-Peptidase 7 (DPP7))
Reactivity
All reactivities for DPP7 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human DPPII (QPP/DPP7) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes Human DPPII. Other species not yet determined.
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for DPP7
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Target
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DPP7
(Dipeptidyl-Peptidase 7 (DPP7))
Alternative Name
DPPII (DPP7 Products )
Synonyms
DPP2 ELISA Kit, DPPII ELISA Kit, QPP ELISA Kit, Dpp2 ELISA Kit, wu:fb19g02 ELISA Kit, zgc:112274 ELISA Kit, zgc:113564 ELISA Kit, DPP7 ELISA Kit, MGC151556 ELISA Kit, dipeptidyl peptidase 7 ELISA Kit, dipeptidylpeptidase 7 ELISA Kit, dipeptidyl-peptidase 7 ELISA Kit, DPP7 ELISA Kit, Dpp7 ELISA Kit, Shewana3_0712 ELISA Kit, Kkor_1052 ELISA Kit, Riean_0388 ELISA Kit, dpp7 ELISA Kit, Isop_1372 ELISA Kit
Gene ID
29952
UniProt
Q9UHL4
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