Dynamitin ELISA Kit

Catalog No. ABIN4882620

Product Details Dynamitin ELISA Kit

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Dynactin subunit 2/DCTN2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Protocadherin-17.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Dynamitin

Target: Dynamitin (DCTN2) (Dynactin 2 (p50) (DCTN2))

Alternative Name: Dynactin Subunit 2 (DCTN2 Products)

Synonyms: DCTN2 ELISA Kit, dctn2 ELISA Kit, dctn50 ELISA Kit, dynamitin ELISA Kit, rbp50 ELISA Kit, CG8269 ELISA Kit, DMN ELISA Kit, Dmel\\CG8269 ELISA Kit, P50 ELISA Kit, dDyn ELISA Kit, dmn ELISA Kit, dyna ELISA Kit, l(2)k16109 ELISA Kit, l(2)k16218 ELISA Kit, p50 ELISA Kit, p50/Dmn ELISA Kit, p50/dynamitin ELISA Kit, 2310042E05Rik ELISA Kit, C130077D06Rik ELISA Kit, C87049 ELISA Kit, DCTN-50 ELISA Kit, GMP23-48K ELISA Kit, RBP50 ELISA Kit, DCTN50 ELISA Kit, DYNAMITIN ELISA Kit, zgc:63867 ELISA Kit, dynactin subunit 2 ELISA Kit, dynactin subunit 2 S homeolog ELISA Kit, Dynactin 2, p50 subunit ELISA Kit, dynactin 2 ELISA Kit, dynactin 2 (p50) ELISA Kit, dynactin subunit 2 L homeolog ELISA Kit, DCTN2 ELISA Kit, dctn2.S ELISA Kit, DCTN2-p50 ELISA Kit, dctn2 ELISA Kit, Dctn2 ELISA Kit, dctn2.L ELISA Kit

Gene ID: 10540

UniProt: Q13561

Pathways: M Phase, Ribonucleoprotein Complex Subunit Organization