×
For best experience we recommend to activate Javascript in your browser.
PTPRJ ELISA Kit
PTPRJ
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details PTPRJ ELISA Kit
(hide)
Target
See all PTPRJ ELISA Kits
PTPRJ
(Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))
Reactivity
All reactivities for PTPRJ elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human DEP-1 (CD148) ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Featured
Discover our best selling PTPRJ ELISA Kit
Top Product
Discover our top product PTPRJ ELISA Kit
Alternatives
(show)
Application Details
(hide)
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
(hide)
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PTPRJ
(hide)
Target
See all PTPRJ ELISA Kits
PTPRJ
(Protein tyrosine Phosphatase, Receptor Type, J (PTPRJ))
Alternative Name
DEP-1 (PTPRJ Products )
Synonyms
HAAP ELISA Kit, CD148 ELISA Kit, DEP1 ELISA Kit, HPTPeta ELISA Kit, R-PTP-ETA ELISA Kit, SCC1 ELISA Kit, AI450271 ELISA Kit, BET ELISA Kit, Byp ELISA Kit, DEP-1 ELISA Kit, PTPbeta2 ELISA Kit, Ptpb2 ELISA Kit, RPTPJ ELISA Kit, Scc-1 ELISA Kit, Scc1 ELISA Kit, PTPRJ ELISA Kit, acid phosphatase 1 ELISA Kit, protein tyrosine phosphatase, receptor type J ELISA Kit, protein tyrosine phosphatase, receptor type, J ELISA Kit, ACP1 ELISA Kit, PTPRJ ELISA Kit, Ptprj ELISA Kit, EUBREC_2762 ELISA Kit
Gene ID
5795
UniProt
Q12913
Pathways
EGFR Signaling Pathway , Platelet-derived growth Factor Receptor Signaling
Recently viewed
(hide)