CSTB ELISA Kit

Catalog No. ABIN4882533

Product Details CSTB ELISA Kit

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Cystatin B (CSTB) ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: The antibody pair provided in this kit recognizes Human Cystatin B (CSTB)

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: Cystatin B (CSTB Products)

Synonyms: CST6 ELISA Kit, EPM1 ELISA Kit, EPM1A ELISA Kit, PME ELISA Kit, STFB ELISA Kit, ULD ELISA Kit, CSTB ELISA Kit, Cyb ELISA Kit, Epm1 ELISA Kit, Stfb ELISA Kit, MGC189005 ELISA Kit, pme ELISA Kit, cst6 ELISA Kit, epm1 ELISA Kit, stfb ELISA Kit, AA960480 ELISA Kit, ARABIDOPSIS THALIANA PHYTOCYSTATIN 6 ELISA Kit, ATCYS6 ELISA Kit, ATCYSB ELISA Kit, cystatin B ELISA Kit, LOC100008600 ELISA Kit, LOC100136235 ELISA Kit, CPI1 ELISA Kit, cystatin B ELISA Kit, cystatin B (stefin B) ELISA Kit, cystatin 14a, tandem duplicate 2 ELISA Kit, cystatin-B ELISA Kit, CSTB ELISA Kit, Cstb ELISA Kit, cstb ELISA Kit, cst14a.2 ELISA Kit, CYSB ELISA Kit, cpi1 ELISA Kit, LOC101123265 ELISA Kit

Gene ID: 1476

UniProt: P04080

Pathways: Response to Water Deprivation