PPIA ELISA Kit

Catalog No. ABIN4882518

Product Details PPIA ELISA Kit

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2.05-500 ng/mL

Minimum Detection Limit: 2.05 ng/mL

Application: ELISA

Purpose: Human Cyclophilin A ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human Cyclophilin. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: Cyclophilin A (PPIA Products)

Synonyms: Cyp1 ELISA Kit, PPIA-2 ELISA Kit, fa93g09 ELISA Kit, ppia ELISA Kit, wu:fa93g09 ELISA Kit, wu:fb05e11 ELISA Kit, wu:fb13h02 ELISA Kit, wu:fb15a05 ELISA Kit, CYPA ELISA Kit, CypA ELISA Kit, Cypa ELISA Kit, CYPH ELISA Kit, CYCA ELISA Kit, CyP-A ELISA Kit, MGC75715 ELISA Kit, 2700098C05 ELISA Kit, Cphn ELISA Kit, CyP-18 ELISA Kit, MT-ND1 ELISA Kit, MTND1 ELISA Kit, NADH1 ELISA Kit, ND1 ELISA Kit, cypA ELISA Kit, PPIase A ELISA Kit, ppial ELISA Kit, wu:fj18g05 ELISA Kit, zgc:73102 ELISA Kit, zgc:86688 ELISA Kit, peptidylprolyl isomerase A ELISA Kit, peptidylprolyl isomerase Aa (cyclophilin A) ELISA Kit, peptidylprolyl isomerase A (cyclophilin A) L homeolog ELISA Kit, cyclophilin A ELISA Kit, cyclophilin a ELISA Kit, peptidylprolyl isomerase A (cyclophilin A) ELISA Kit, peptidylprolyl isomerase Ab (cyclophilin A) ELISA Kit, PPIA ELISA Kit, ppiaa ELISA Kit, ppia.L ELISA Kit, Cypa ELISA Kit, CNB01230 ELISA Kit, CNB01290 ELISA Kit, Tc00.1047053506925.300 ELISA Kit, Tb11.03.0250 ELISA Kit, CYPA ELISA Kit, Ppia ELISA Kit, ppia ELISA Kit, ppiab ELISA Kit

Gene ID: 5478

UniProt: P62937