c-MYC ELISA Kit

Catalog No. ABIN4882399

Product Details c-MYC ELISA Kit

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-150 ng/mL

Minimum Detection Limit: 0.62 ng/mL

Application: ELISA

Purpose: Human c-Myc (MYC) ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human c-Myc. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc///MYC (MYC Products)

Synonyms: MRTL ELISA Kit, MYCC ELISA Kit, bHLHe39 ELISA Kit, c-Myc ELISA Kit, CMYC ELISA Kit, c-myc ELISA Kit, C-MYC ELISA Kit, LOC100136746 ELISA Kit, ARC ELISA Kit, B430311C09Rik ELISA Kit, MYC ELISA Kit, NOP ELISA Kit, Nop30 ELISA Kit, RNCMYC ELISA Kit, mMyc ELISA Kit, AU016757 ELISA Kit, Myc2 ELISA Kit, Niard ELISA Kit, Nird ELISA Kit, cmyc ELISA Kit, zc-myc ELISA Kit, c-myc II ELISA Kit, myc-B ELISA Kit, myc2 ELISA Kit, myc1 ELISA Kit, CMyc ELISA Kit, zgc:55680 ELISA Kit, CG10798 ELISA Kit, D-Myc ELISA Kit, DM ELISA Kit, DMYc ELISA Kit, Dm ELISA Kit, Dmel\CG10798 ELISA Kit, Dmyc ELISA Kit, EG:BACN5I9.1 ELISA Kit, anon-WO03040301.171 ELISA Kit, bHLHe57 ELISA Kit, c-MYC ELISA Kit, d-myc ELISA Kit, dMYC ELISA Kit, dMyc ELISA Kit, dMyc1 ELISA Kit, da ELISA Kit, dm ELISA Kit, dm/dMyc ELISA Kit, dm/myc ELISA Kit, dmyc ELISA Kit, dmyc1 ELISA Kit, l(1)G0139 ELISA Kit, l(1)G0354 ELISA Kit, l(1)G0359 ELISA Kit, myc ELISA Kit, MYC proto-oncogene, bHLH transcription factor ELISA Kit, v-myc myelocytomatosis viral oncogene homolog ELISA Kit, nucleolar protein 3 (apoptosis repressor with CARD domain) ELISA Kit, myelocytomatosis oncogene ELISA Kit, MYC proto-oncogene, bHLH transcription factor a ELISA Kit, MYC proto-oncogene, bHLH transcription factor L homeolog ELISA Kit, MYC proto-oncogene, bHLH transcription factor S homeolog ELISA Kit, v-myc myelocytomatosis viral oncogene homolog (avian) ELISA Kit, MYC proto-oncogene, bHLH transcription factor b ELISA Kit, CG10798 gene product from transcript CG10798-RB ELISA Kit, MYC ELISA Kit, LOC100136746 ELISA Kit, Nol3 ELISA Kit, Myc ELISA Kit, myca ELISA Kit, myc.L ELISA Kit, myc.S ELISA Kit, mycb ELISA Kit

Gene ID: 4609

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect