CD99 ELISA Kit

Catalog No. ABIN4882271

Product Details CD99 ELISA Kit

Target: CD99

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human CD99 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human CD99.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD99

Target: CD99

Alternative Name: CD99 (CD99 Products)

Synonyms: 1110061M03Rik ELISA Kit, 2410026K10Rik ELISA Kit, D4 ELISA Kit, Pilr-l ELISA Kit, pilr-1 ELISA Kit, HBA71 ELISA Kit, MIC2 ELISA Kit, MIC2X ELISA Kit, MIC2Y ELISA Kit, MSK5X ELISA Kit, CD99 antigen ELISA Kit, CD99 molecule (Xg blood group) ELISA Kit, CD99 molecule ELISA Kit, Cd99 ELISA Kit, CD99 ELISA Kit

Target Type: Viral Protein

Gene ID: 4267

UniProt: P14209