CD74 ELISA Kit

Catalog No. ABIN4882248

Product Details CD74 ELISA Kit

Target: CD74 (HLA-DR-gamma (CD74))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.10-25 ng/mL

Minimum Detection Limit: 0.10 ng/mL

Application: ELISA

Purpose: Human CD74 ELISA Kit for serum, plasma, and cell culture supernatant samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair detects human CD74. Other species not determined.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CD74

Target: CD74 (HLA-DR-gamma (CD74))

Alternative Name: CD74 (CD74 Products)

Synonyms: DHLAG ELISA Kit, HLADG ELISA Kit, II ELISA Kit, Ia-GAMMA ELISA Kit, CLIP ELISA Kit, Ii ELISA Kit, INVG34 ELISA Kit, Iclp-1 ELISA Kit, iclp1 ELISA Kit, wu:fb11f09 ELISA Kit, wu:fb12a01 ELISA Kit, wu:fb13d06 ELISA Kit, wu:fk94e07 ELISA Kit, Iclp-2 ELISA Kit, cd74 ELISA Kit, iclp2 ELISA Kit, zgc:123161 ELISA Kit, CD74 molecule ELISA Kit, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) ELISA Kit, CD74 molecule, major histocompatibility complex, class II invariant chain a ELISA Kit, CD74 molecule, major histocompatibility complex, class II invariant chain b ELISA Kit, CD74 ELISA Kit, Cd74 ELISA Kit, cd74a ELISA Kit, cd74b ELISA Kit

Gene ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints