CNTNAP2 ELISA Kit

Catalog No. ABIN4882106

Product Details CNTNAP2 ELISA Kit

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Caspr2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CNTNAP2

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Alternative Name: Caspr2 (CNTNAP2 Products)

Synonyms: AUTS15 ELISA Kit, CASPR2 ELISA Kit, CDFE ELISA Kit, NRXN4 ELISA Kit, PTHSL1 ELISA Kit, 5430425M22Rik ELISA Kit, Caspr2 ELISA Kit, mKIAA0868 ELISA Kit, CASPR ELISA Kit, MGC145769 ELISA Kit, MGC147944 ELISA Kit, MGC147981 ELISA Kit, contactin associated protein like 2 ELISA Kit, contactin associated protein-like 2 ELISA Kit, contactin associated protein like 2b ELISA Kit, CNTNAP2 ELISA Kit, Cntnap2 ELISA Kit, cntnap2b ELISA Kit, cntnap2 ELISA Kit

Gene ID: 26047

UniProt: Q9UHC6