CA5B ELISA Kit

Catalog No. ABIN4882055

Product Details CA5B ELISA Kit

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human CA5B ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for CA5B

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: CA5B (CA5B Products)

Synonyms: CA-VB ELISA Kit, 7330410H16Rik ELISA Kit, CAVB ELISA Kit, Ca5b ELISA Kit, CarVb ELISA Kit, D730005F19Rik ELISA Kit, zgc:171653 ELISA Kit, CA5B ELISA Kit, ca5b ELISA Kit, carbonic anhydrase 5B ELISA Kit, carbonic anhydrase 5b, mitochondrial ELISA Kit, carbonic anhydrase Va ELISA Kit, carbonic anhydrase 5A ELISA Kit, carbonic anhydrase 5B, mitochondrial ELISA Kit, carbonic anhydrase VB, mitochondrial L homeolog ELISA Kit, CA5B ELISA Kit, Car5b ELISA Kit, Ca5b ELISA Kit, ca5a ELISA Kit, CA5A ELISA Kit, LOC100051095 ELISA Kit, ca5b.L ELISA Kit

Gene ID: 11238

UniProt: Q9Y2D0