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BMPR2 ELISA Kit
BMPR2
Reactivity: Human
Colorimetric
Sandwich ELISA
2.5-600 ng/mL
Cell Culture Supernatant, Plasma, Serum
Product Details BMPR2 ELISA Kit
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Target
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BMPR2
(Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))
Reactivity
All reactivities for BMPR2 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
2.5-600 ng/mL
Minimum Detection Limit
2.5 ng/mL
Application
ELISA
Purpose
Human BMPR-II ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Sample Type
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificity
The antibody pair provided in this kit recognizes Human BMPR-2
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for BMPR2
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Target
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BMPR2
(Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2))
Alternative Name
BMPR-II (BMPR2 Products )
Synonyms
BMPR-II ELISA Kit, BMPR3 ELISA Kit, BMR2 ELISA Kit, BRK-3 ELISA Kit, PPH1 ELISA Kit, T-ALK ELISA Kit, 2610024H22Rik ELISA Kit, AL117858 ELISA Kit, AW546137 ELISA Kit, BB189135 ELISA Kit, BMP-2 ELISA Kit, BMPR-2 ELISA Kit, BMPRII ELISA Kit, Gm20272 ELISA Kit, Bmpr-II ELISA Kit, bmprii ELISA Kit, bone morphogenetic protein receptor type 2 ELISA Kit, bone morphogenetic protein receptor, type II b (serine/threonine kinase) ELISA Kit, bone morphogenetic protein receptor, type II (serine/threonine kinase) ELISA Kit, BMPR2 ELISA Kit, bmpr2b ELISA Kit, Bmpr2 ELISA Kit
Gene ID
659
UniProt
Q13873
Pathways
Growth Factor Binding
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