ICOSLG ELISA Kit

Catalog No. ABIN4881923

Product Details ICOSLG ELISA Kit

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.41-100 ng/mL

Minimum Detection Limit: 0.41 ng/mL

Application: ELISA

Purpose: Human B7-H2/ICOS Ligand ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human B7-H2.

Sensitivity: 0.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Alternative Name: B7-H2 (ICOSLG Products)

Synonyms: ICOSLG ELISA Kit, AU044799 ELISA Kit, B7-H2 ELISA Kit, B7RP-1 ELISA Kit, B7h ELISA Kit, BG071784 ELISA Kit, GI50 ELISA Kit, GL50 ELISA Kit, GL50-B ELISA Kit, ICOS-L ELISA Kit, Icoslg ELISA Kit, LICOS ELISA Kit, Ly115l ELISA Kit, mKIAA0653 ELISA Kit, B7H2 ELISA Kit, B7RP1 ELISA Kit, CD275 ELISA Kit, ICOSL ELISA Kit, Icosl ELISA Kit, RGD1562791 ELISA Kit, inducible T-cell costimulator ligand ELISA Kit, icos ligand ELISA Kit, inducible T-cell co-stimulator ligand ELISA Kit, ICOSLG ELISA Kit, Icosl ELISA Kit, Icoslg ELISA Kit

Gene ID: 10272399623308

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation