Annexin V ELISA Kit

Catalog No. ABIN4881843

Product Details Annexin V ELISA Kit

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human Annexin A5/Annexin V ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human Cathepsin C.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for Annexin V

Target: Annexin V (ANXA5) (Annexin A5 (ANXA5))

Alternative Name: Annexin A5 (ANXA5 Products)

Synonyms: anx ELISA Kit, anx5 ELISA Kit, ANX V ELISA Kit, anxa5 ELISA Kit, cb989 ELISA Kit, wu:fa98f06 ELISA Kit, wu:fj10f10 ELISA Kit, MGC89158 ELISA Kit, ANX5 ELISA Kit, ENX2 ELISA Kit, PP4 ELISA Kit, RPRGL3 ELISA Kit, Anx5 ELISA Kit, R74653 ELISA Kit, LC5 ELISA Kit, enx2 ELISA Kit, annexin A5 ELISA Kit, annexin A5b ELISA Kit, Annexin A5 ELISA Kit, annexin A5 L homeolog ELISA Kit, ANXA5 ELISA Kit, anxa5b ELISA Kit, anxa5 ELISA Kit, Anxa5 ELISA Kit, anxa5.L ELISA Kit

Gene ID: 308

UniProt: P08758

Pathways: Apoptosis