BMPR1B ELISA Kit

Catalog No. ABIN4881803

Product Details BMPR1B ELISA Kit

Target: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human ALK-6/BMPR-IB ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human ALK-6.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for BMPR1B

Target: BMPR1B (Bone Morphogenetic Protein Receptor, Type IB (BMPR1B))

Alternative Name: ALK-6 (BMPR1B Products)

Synonyms: ALK6 ELISA Kit, BMPR-IB ELISA Kit, FecB ELISA Kit, alk-6 ELISA Kit, alk6 ELISA Kit, cdw293 ELISA Kit, alk6b ELISA Kit, zgc:172219 ELISA Kit, BMPR1B ELISA Kit, AI385617 ELISA Kit, ALK-6 ELISA Kit, AV355320 ELISA Kit, Acvrlk6 ELISA Kit, Alk6 ELISA Kit, BMPR-1B ELISA Kit, CFK-43a ELISA Kit, SKR6 ELISA Kit, BR1b ELISA Kit, alk6tr ELISA Kit, bmpr1b ELISA Kit, zALK-6 ELISA Kit, zgc:92220 ELISA Kit, CDw293 ELISA Kit, BMP15 ELISA Kit, BMPRIB ELISA Kit, RPK-1 ELISA Kit, bone morphogenetic protein receptor type 1B ELISA Kit, bone morphogenetic protein receptor, type IBb ELISA Kit, bone morphogenetic protein receptor, type 1B ELISA Kit, bone morphogenetic protein receptor, type IBa ELISA Kit, BMPR1B ELISA Kit, bmpr1b ELISA Kit, bmpr1bb ELISA Kit, Bmpr1b ELISA Kit, bmpr1ba ELISA Kit

Gene ID: 658

UniProt: O00238