ALDH1A1 ELISA Kit

Catalog No. ABIN4881794

Product Details ALDH1A1 ELISA Kit

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human ALDH1A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for ALDH1A1

Target: ALDH1A1 (Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))

Alternative Name: ALDH1A1 (ALDH1A1 Products)

Synonyms: ALDC ELISA Kit, ALDH-E1 ELISA Kit, ALDH1 ELISA Kit, ALDH11 ELISA Kit, PUMB1 ELISA Kit, RALDH1 ELISA Kit, AL1A1 ELISA Kit, aldc ELISA Kit, aldh-e1 ELISA Kit, aldh1 ELISA Kit, aldh11 ELISA Kit, pumb1 ELISA Kit, raldh1 ELISA Kit, GGADHR ELISA Kit, ALDH1A1 ELISA Kit, ALDDH ELISA Kit, Ahd2 ELISA Kit, Aldh1 ELISA Kit, Aldh2 ELISA Kit, Ahd-2 ELISA Kit, Aldh1a2 ELISA Kit, E1 ELISA Kit, Raldh1 ELISA Kit, Aldh1a1 ELISA Kit, ALHDII ELISA Kit, RALDH 1 ELISA Kit, RalDH1 ELISA Kit, aldehyde dehydrogenase 1 family member A1 ELISA Kit, aldehyde dehydrogenase 1 family, member A1 ELISA Kit, aldehyde dehydrogenase 1 family member A1 L homeolog ELISA Kit, aldehyde dehydrogenase family 1, subfamily A1 ELISA Kit, aldehyde dehydrogenase ELISA Kit, retinal dehydrogenase 1 ELISA Kit, ALDH1A1 ELISA Kit, aldh1a1 ELISA Kit, Aldh1a1 ELISA Kit, aldh1a1.L ELISA Kit, aldH1 ELISA Kit, LOC100732581 ELISA Kit, LOC101822890 ELISA Kit

Gene ID: 216

UniProt: P00352

Pathways: Dopaminergic Neurogenesis