AHCY ELISA Kit

Catalog No. ABIN4881774

Product Details AHCY ELISA Kit

Target: AHCY (Adenosylhomocysteinase (AHCY))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human AHCY ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human APE1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for AHCY

Target: AHCY (Adenosylhomocysteinase (AHCY))

Alternative Name: AHCY (AHCY Products)

Synonyms: sahh ELISA Kit, PSPTO5068 ELISA Kit, Ahcy ELISA Kit, SAHH ELISA Kit, adoHcyase ELISA Kit, ahcy ELISA Kit, AA987153 ELISA Kit, AL024110 ELISA Kit, CuBP ELISA Kit, D150 ELISA Kit, cb1079 ELISA Kit, hm:zeh1173 ELISA Kit, hm:zeh1364 ELISA Kit, wu:fj67b02 ELISA Kit, adenosylhomocysteinase ELISA Kit, Adenosylhomocysteinase ELISA Kit, S-adenosylhomocysteine hydrolase ELISA Kit, adenosylhomocysteinase S homeolog ELISA Kit, AHCY ELISA Kit, ahcy ELISA Kit, ahcY ELISA Kit, MMAH_RS02605 ELISA Kit, Srot_2217 ELISA Kit, Ndas_3755 ELISA Kit, Deba_0936 ELISA Kit, Palpr_2392 ELISA Kit, Calni_0569 ELISA Kit, LOC100282150 ELISA Kit, Intca_2510 ELISA Kit, Marky_0392 ELISA Kit, Desac_1394 ELISA Kit, Tc00.1047053511229.50 ELISA Kit, Tc00.1047053511589.200 ELISA Kit, Tb11.01.1350 ELISA Kit, LMJF_36_3910 ELISA Kit, MCYG_06244 ELISA Kit, Ahcy ELISA Kit, ahcy.S ELISA Kit

Gene ID: 191

UniProt: P23526