Substance P ELISA Kit

Catalog No. ABIN4881333

Product Details Substance P ELISA Kit

Target: Substance P

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1-1.000 ng/mL

Minimum Detection Limit: 0.1 ng/mL

Application: ELISA

Purpose: Human/Mouse/Rat Substance P EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat Substance P.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Application Notes: Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 8X

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for Substance P

Target: Substance P

Abstract: Substance P Products

Synonyms: Hs.2563 ELISA Kit, NK2 ELISA Kit, NKNA ELISA Kit, NPK ELISA Kit, TAC2 ELISA Kit, PPTA3 ELISA Kit, Ppt5fl ELISA Kit, RATPPTA3 ELISA Kit, TAC ELISA Kit, 4930528L02Rik ELISA Kit, NK-1 ELISA Kit, NK1 ELISA Kit, Nkna ELISA Kit, PPT-A ELISA Kit, PPTA ELISA Kit, SP ELISA Kit, PPT ELISA Kit, beta-PPT-A ELISA Kit, NKA ELISA Kit, tachykinin precursor 1 ELISA Kit, tachykinin, precursor 1 ELISA Kit, tachykinin 1 ELISA Kit, TAC1 ELISA Kit, Tac1 ELISA Kit

Gene ID: 6863

UniProt: P20366